Development of Phaseolus vulgaris endogenous reference and Embrapa 5.1 event-specific assays for quantification of Embrapa 5.1 GM common bean using real time PCR
نویسندگان
چکیده
Background Brazil is the second largest producer of genetically modified (GM) crops with 37 GM crop varieties approved by CTNBio for commercialization. One of these varieties is the Embrapa 5.1 common bean, resistant to the bean golden mosaic virus (BGMV). The Embrapa 5.1 event was developed using the RNAi concept to induce the silencing of the AC1 viral gene [1]. Labelling of food products containing GMOs in Brazil is mandatory [2], so methods for quantification of GMOs are required. Real time PCR methods have been used as the gold standard for GMO quantification. In a previous work, we have developed a construct-specific PCR detection assay for Embrapa 5.1 [3]. In this study we developed an event-specific PCR assay for Embrapa 5.1.
منابع مشابه
Primers, probes and plasmid development for specific PCR detection of genetically modified common bean (Phaseolus vulgaris) Embrapa 5.1
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